Advertisement

 

 

A cell-based high-throughput protocol to screen entry inhibitors of highly pathogenic viruses with Traditional Chinese Medicines.

A cell-based high-throughput protocol to screen entry inhibitors of highly pathogenic viruses with Traditional Chinese Medicines.
Author Information (click to view)

Yang Y, Cheng H, Yan H, Wang PZ, Rong R, Zhang YY, Zhang CB, Du RK, Rong LJ,


Yang Y, Cheng H, Yan H, Wang PZ, Rong R, Zhang YY, Zhang CB, Du RK, Rong LJ, (click to view)

Yang Y, Cheng H, Yan H, Wang PZ, Rong R, Zhang YY, Zhang CB, Du RK, Rong LJ,

Advertisement
Share on FacebookTweet about this on TwitterShare on LinkedIn

Journal of medical virology 2016 Oct 5() doi 10.1002/jmv.24705

Abstract

Emerging viruses such as Ebola virus (EBOV), Lassa virus (LASV) and avian influenza virus H5N1 (AIV) are global health concerns. Since there is very limited options (either vaccine or specific therapy) approved for humans against these viruses, there is an urgent need to develop prophylactic and therapeutic treatments. Previously we reported a high-throughput screening (HTS) protocol to identify entry inhibitors for three highly pathogenic viruses (EBOV, LASV, and AIV) using a human immunodeficiency virus-based pseudotyping platform which allows us to perform the screening in a BSL-2 facility. In this report, we have adopted this screening protocol to evaluate traditional Chinese Medicines (TCMs) in an effort to discover entry inhibitors against these viruses. Here we show that extracts of the following Chinese medicinal herbs exhibit potent anti-Ebola viral activities: Gardenia jasminoides Ellis, Citrus aurantium L., Viola yedoensis Makino, Prunella vulgaris L., Coix lacryma-jobi L. var. mayuen (Roman.) Stapf, Pinellia ternata (Thunb.) Breit. and Morus alba L.. This study represents a proof-of-principle investigation supporting the suitability of this assay for rapid screening TCMs and identifying putative entry inhibitors for these viruses. This article is protected by copyright. All rights reserved.

Submit a Comment

Your email address will not be published. Required fields are marked *

fourteen + three =

[ HIDE/SHOW ]