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Cryptococcus neoformans population diversity and clinical outcomes of HIV-associated cryptococcal meningitis patients in Zimbabwe.

Cryptococcus neoformans population diversity and clinical outcomes of HIV-associated cryptococcal meningitis patients in Zimbabwe.
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Nyazika TK, Hagen F, Machiridza T, Kutepa M, Masanganise F, Hendrickx M, Boekhout T, Magombei-Majinjiwa T, Siziba N, Chin'ombe N, Mateveke K, Meis J, Robertson VJ,


Nyazika TK, Hagen F, Machiridza T, Kutepa M, Masanganise F, Hendrickx M, Boekhout T, Magombei-Majinjiwa T, Siziba N, Chin'ombe N, Mateveke K, Meis J, Robertson VJ, (click to view)

Nyazika TK, Hagen F, Machiridza T, Kutepa M, Masanganise F, Hendrickx M, Boekhout T, Magombei-Majinjiwa T, Siziba N, Chin'ombe N, Mateveke K, Meis J, Robertson VJ,

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Journal of medical microbiology 2016 9 13() doi 10.1099/jmm.0.000354

Abstract

HIV and cryptococcal meningitis coinfection are a major public health problem in most developing countries. Cryptococcus neoformans sensu stricto is responsible for the majority of HIV-associated cryptococcosis cases in sub-Saharan Africa. Despite the available information, little is known about cryptococcal population diversity and its association with clinical outcomes in patients with HIV-associated cryptococcal meningitis in sub-Saharan Africa. In a prospective cohort we investigated the prevalence and clinical outcome of C. neoformans sensu stricto meningitis among HIV-infected patients in Harare, Zimbabwe and compared the genotypic diversity of the isolates with those collected from other parts of Africa. Molecular typing was done using amplified fragment length polymorphism genotyping and microsatellite typing. The majority of patients with HIV-associated C. neoformans sensu stricto meningitis in this cohort were males (n=33/55; 60.0%). The predominant C. neoformans sensu stricto genotype among the Zimbabwean isolates was genotype AFLP1/VNI (n=40; 72.7%), followed by AFLP1A/VNB/VNII (n=8; 14.6%) and AFLP1B/VNII was the least isolated (n=7; 12.7%). Most of the isolates were mating-type α (n=51; 92.7%) and only 4 (7.3%) were mating-type a. Overall in-hospital mortality was 55.6% (n=30) and no difference between infecting genotype and clinical outcome of patient (P=0.73) or CD4+ counts (P=0.79) was observed. Zimbabwean C. neoformans sensu stricto genotypes demonstrated a high level of genetic diversity by microsatellite typing and 51 genotypes within the main molecular types AFLP1/VNI, AFLP1A/VNB/VNII and AFLP1B/VNII were identified. This study demonstrate that C. neoformans sensu stricto in Zimbabwe has a high level of genetic diversity when compared to other regional isolates.

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