Advertisement

 

 

Fluorescent protein nanowire-mediated protein microarrays for multiplexed and highly sensitive pathogen detection.

Author Information (click to view)

Men D, Zhou J, Li W, Leng Y, Chen X, Tao SC, Zhang XE,


Men D, Zhou J, Li W, Leng Y, Chen X, Tao SC, Zhang XE, (click to view)

Men D, Zhou J, Li W, Leng Y, Chen X, Tao SC, Zhang XE,

Advertisement
Share on FacebookTweet about this on TwitterShare on LinkedIn

ACS applied materials & interfaces 2016 6 17()

Abstract

Protein microarrays are powerful tools for high-throughput and simultaneous detection of different target molecules in complex biological samples. However, the sensitivity of conventional fluorescence-labeling protein detection methods is limited by the availability of signal molecules for binding to the target molecule. Here, we built a multi-functional fluorescent protein nanowire (FNw) by harnessing self-assembly of yeast amyloid protein. The FNw integrated a large number of fluorescent molecules, thereby enhancing the fluorescent signal output in target detection. The FNw were then combined with protein microarray technology for detecting proteins derived from two pathogens including influenza virus (hemagglutinin 1, HA1) and human immunodeficiency virus (p24 and gp120). The resulting detection sensitivity achieved a 100-fold improvement over a commercially available detection reagent.

Submit a Comment

Your email address will not be published. Required fields are marked *

two × two =

[ HIDE/SHOW ]