Currently, conventional enzyme immunoassays which use manual gold, and colloidal tests (GICT) are used as a screening tool to detect Treponema pallidum (syphilis), hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency viruses (HIV 1/2) in patients undergoing surgery. The present observational, cross-sectional study compared the sensitivity, specificity and workflow characteristics of the conventional algorithm using manual GICT with a newly proposed algorithm that uses automated BIO-FLASH® technology as a screening tool in patients undergoing gastrointestinal (GI) endoscopy. A total of 956 patients were examined for the presence of serological infection markers (HIV-1/2, HCV, HBV, and syphilis). The proposed algorithm using BIO-FLASH® technology was superior in the detection of all markers (100.0% sensitivity and specificity for anti-HIV and anti-HCV antibodies, HBV surface antigen [HBsAg] and syphilis detection) compared with the conventional algorithm based on the manual method (80.0% sensitivity and 98.6% specificity for anti-HIV; 75.0% sensitivity for anti-HCV and 94.7% for HBsAg, and 100% specificity for both markers) in these patients. The automated BIO-FLASH® screening algorithm also reduced the operation time by 85.0% (205 minutes) per day, saving up to 24 hours/week. In conclusion, the use of the newly proposed screening algorithm based on the automated BIO-FLASH® technology can provide an advantage over the conventional algorithms based on manual methods in the screening of HIV, HBV, HCV and syphilis before GI endoscopy.
HIV, HBV, HCV and syphilis blood testing using BIO-FLASH® technology based algorithm before gastrointestinal endoscopy.