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Laboratory evaluation of a point of care downward flow assay for simultaneous detection of antibodies to Treponema pallidum and human immunodeficiency virus.

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Herbst de Cortina S, Bristow CC, Vargas SK, Perez DG, Konda KA, Caceres CF, Klausner JD,


Herbst de Cortina S, Bristow CC, Vargas SK, Perez DG, Konda KA, Caceres CF, Klausner JD, (click to view)

Herbst de Cortina S, Bristow CC, Vargas SK, Perez DG, Konda KA, Caceres CF, Klausner JD,

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Journal of clinical microbiology 2016 5 4() pii

Abstract

The rapid downward flow assay detected human immunodeficiency virus and Treponema pallidum serum antibodies with sensitivities/specificities of 100% (95% CI: 95.9-100%)/95.5% (95% CI: 89.9-98.5%) and 87.4% (95% CI: 81.4-92.0%)/97.0% (95% CI: 84.2-99.9%), respectively (n=200). The syphilis antibody sensitivity was higher in patients with a rapid plasma reagin titer ≥1:8 (97.3%) versus ≤1:4 (90%) or a non reactive titer (66.7%).

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