Advertisement

 

 

Pilosebaceous Targeting by Isotretenoin Loaded Invasomal Gel for the Treatment of Eosinophilic Pustular Folliculitis: Optimization, efficacy and cellular analysis.

Pilosebaceous Targeting by Isotretenoin Loaded Invasomal Gel for the Treatment of Eosinophilic Pustular Folliculitis: Optimization, efficacy and cellular analysis.
Author Information (click to view)

Dwivedi M, Sharma V, Pathak K,


Dwivedi M, Sharma V, Pathak K, (click to view)

Dwivedi M, Sharma V, Pathak K,

Advertisement
Share on FacebookTweet about this on TwitterShare on LinkedIn

Drug development and industrial pharmacy 2016 9 21() 1-39

Abstract
CONTEXT
Eosinophilic pustular folliculitis is a secondary symptom associated with HIV infection appears as levels of CD4 lymphocyte cells and T4 lymphocyte cell. Isotretinoin, an analogue of vitamin A (retinoid) alters the DNA transcription mechanism and interferes in the process of DNA formation. It also inhibits the eosinophilic chemotactic factors present in sebaceous lipids and in the stratum corneum of patients suffering from this ailment.

OBJECTIVE
The present research was aimed to formulate isotretenoin loaded invasomal gel to deliver and target the drug to pilosebaceous follicular unit.

METHODS
Nine invasomal formulations (F1 – F9) were prepared applying 3(2) factorial designs and characterized.

RESULTS
Formulation F9 was selected as optimized formulation due to optimum results and highest %CDP of 85.94 ± 1.86% in 8hr. TEM suggested uniformity in vesicles shape and size in F9 and developed as invasomal gel (IG).

LIMITATIONS
Clinical phase I, II and III studies will be required before using on human patients.

CONCLUSION
CLSM validate that IG successfully reaches the pilosebaceous follicular unit and further studied on cell line (SZ-95) exhibited IC50 of ≤ 8 (25µM of isotretenoin). Cell cycle analysis confirmed IG arrested the cell growth up to 82% with insignificant difference to pure isotretenion.

Submit a Comment

Your email address will not be published. Required fields are marked *

1 × 2 =

[ HIDE/SHOW ]