Advertisement

 

 

Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array.

Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array.
Author Information (click to view)

Lee J, Hong MH, Han S, Na J, Kim I, Kwon YJ, Lim YB, Choi HJ,


Lee J, Hong MH, Han S, Na J, Kim I, Kwon YJ, Lim YB, Choi HJ, (click to view)

Lee J, Hong MH, Han S, Na J, Kim I, Kwon YJ, Lim YB, Choi HJ,

Advertisement
Share on FacebookTweet about this on TwitterShare on LinkedIn

Nanoscale research letters 2016 07 2211(1) 341 doi 10.1186/s11671-016-1504-8

Abstract

In this study, HIV-1 Rev response element (RRE) RNA was detected via an Au-coated vertical silicon nanowire electrode array (VSNEA). The VSNEA was fabricated by combining bottom-up and top-down approaches and then immobilized by artificial peptides for the recognition of HIV-1 RRE. Differential pulse voltammetry (DPV) analysis was used to measure the electrochemical response of the peptide-immobilized VSNEA to the concentration and types of HIV-1 RRE RNA. DPV peaks showed linearity to the concentration of RNA with a detection limit down to 1.513 fM. It also showed the clear different peaks to the mutated HIV-1 RRE RNA. The high sensitivity and selectivity of VSNEA for the detection of HIV-1 RRE RNA may be attributed to the high surface-to-volume ratio and total overlap diffusion mode of ions of the one-dimensional nanowire electrodes.

Submit a Comment

Your email address will not be published. Required fields are marked *

seventeen + four =

[ HIDE/SHOW ]