For a study, researchers sought to collect high-resolution anorectal manometry (HRAM) data from healthy newborns in general, as well as subgroups of infants with functional gastrointestinal disorders (FGD) functional constipation (FC), infant colic (IC), and infant dyschezia (ID) (ROME 3 criteria). Healthy term infants with HRAM at 2 and 6 months were included in the prospective observational cohort trial, which used a water-perfused pediatric anorectal silicone catheter. The presence of anorectal waves and the rectoanal inhibitory reflex (RAIR) and the volume required to trigger RAIR, and the anal resting pressure (ARP) were all recorded. Subjects with FC, IC, and ID were identified using questionnaires at 2 and 6 months. About 187 HRAM examinations were performed on 107 newborns with no problems. At least once, normal RAIR was discovered in all of them. In infants without FGD, mean ARP increased between 2- and 6-month investigations. There were no notable variations in HRAM data between infants with and without an FGD. FC, IC, and ID were seen at 2 months (2%, 4%, and 17%, respectively), but only FC and ID were seen at 6 months (7% and 1%). Investigators had data on HRAM in infants aged 2 and 6 months. RAIR was discovered in all healthy neonates who had repeated HRAM. HRAM was a safe, acceptable treatment option for newborns with or without FGD.