Advertisement

 

 

A rapid diagnostic workflow for cefotaxime-resistant Escherichia coli and Klebsiella pneumoniae detection from blood cultures by MALDI-TOF mass spectrometry.

A rapid diagnostic workflow for cefotaxime-resistant Escherichia coli and Klebsiella pneumoniae detection from blood cultures by MALDI-TOF mass spectrometry.
Author Information (click to view)

De Carolis E, Paoletti S, Nagel D, Vella A, Mello E, Palucci I, De Angelis G, D'Inzeo T, Sanguinetti M, Posteraro B, Spanu T,


De Carolis E, Paoletti S, Nagel D, Vella A, Mello E, Palucci I, De Angelis G, D'Inzeo T, Sanguinetti M, Posteraro B, Spanu T, (click to view)

De Carolis E, Paoletti S, Nagel D, Vella A, Mello E, Palucci I, De Angelis G, D'Inzeo T, Sanguinetti M, Posteraro B, Spanu T,

Advertisement

PloS one 2017 10 0512(10) e0185935 doi 10.1371/journal.pone.0185935
Abstract
BACKGROUND
Nowadays, the global spread of resistance to oxyimino-cephalosporins in Enterobacteriaceae implies the need for novel diagnostics that can rapidly target resistant organisms from these bacterial species.

METHODS
In this study, we developed and evaluated a Direct Mass Spectrometry assay for Beta-Lactamase (D-MSBL) that allows direct identification of (oxyimino)cephalosporin-resistant Escherichia coli or Klebsiella pneumoniae from positive blood cultures (BCs), by using the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technology.

RESULTS
The D-MSBL assay was performed on 93 E. coli or K. pneumoniae growing BC samples that were shortly co-incubated with cefotaxime (CTX) as the indicator cephalosporin. Susceptibility and resistance defining peaks from the samples’ mass spectra were analyzed by a novel algorithm for bacterial organism classification. The D-MSBL assay allowed discrimination between E. coli and K. pneumoniae that were resistant or susceptible to CTX with a sensitivity of 86.8% and a specificity of 98.2%.

CONCLUSION
The proposed algorithm-based D-MSBL assay, if integrated in the routine laboratory diagnostic workflow, may be useful to enhance the establishment of appropriate antibiotic therapy and to control the threat of oxyimino-cephalosporin resistance in hospital.

Submit a Comment

Your email address will not be published. Required fields are marked *

eighteen − four =

[ HIDE/SHOW ]