Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies that causes problems in female fertility at the reproductive age. PCOS is a multifactorial disease, with genetic factors playing a crucial role in its development. H19 is a long non-coding RNA (lncRNA) expressed from the maternal chromosome, which is correlated with PCOS.
In this study, 115 women suffering from PCOS and 130 healthy women with regular menstrual cycles were recruited as case and control groups, respectively. After the extraction of genomic DNA, the restriction fragment length polymorphism polymerase chain reaction was employed for genotyping of rs2067051G > A and rs3741219T > C. Statistical analysis was done using SPSS package V.22 for Windows. In silico analysis was recruited to determine the effects of SNPs on the secondary structure of gene transcript as well as miRNA binding sites.
The obtained data showed that the A allele of rs2067051G > A was associated with the high risk of polycystic ovary syndrome (OR = 2.00, 95%CI = 1.38-2.91, p = 0.00). AG and AA genotypes led to a 3.64- and (about) a five-fold increase in the risk of PCOS, respectively (95%CI = 2.02-6.54, p = 0.00, and 95%CI = 1.51-16.52, p = 0.00, respectively). These variants caused a significant increase in the risk of this disorder in all genotype models except in the recessive model. However, no association was found between rs3741219T > C and the increased risk of PCOS, either in the allele or in the genotype models.
According to the findings, rs2067051G > A is associated with an increased risk of polycystic ovary syndrome in the Iranian population.

© The Author(s) 2020. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

References

PubMed