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Analysis of cytomegalovirus-specific T-cell responses in patients with hypertension: comparison of assay methods and antigens.

Analysis of cytomegalovirus-specific T-cell responses in patients with hypertension: comparison of assay methods and antigens.
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Youn JC, Kim JY, Jung MK, Yu HT, Park SH, Kim IC, Lee SK, Choi SW, Han S, Ryu KH, Park S, Shin EC,


Youn JC, Kim JY, Jung MK, Yu HT, Park SH, Kim IC, Lee SK, Choi SW, Han S, Ryu KH, Park S, Shin EC, (click to view)

Youn JC, Kim JY, Jung MK, Yu HT, Park SH, Kim IC, Lee SK, Choi SW, Han S, Ryu KH, Park S, Shin EC,

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Clinical hypertension 2018 03 2024() 5 doi 10.1186/s40885-018-0090-8
Abstract
Background
Recent studies suggest an association between cytomegalovirus (CMV) infection and hypertension. In the present study, we used a variety of antigens and different assay methods to investigate the relationship between CMV-specific T-cell responses and arterial stiffness in patients with hypertension.

Methods
To evaluate arterial stiffness, pulse wave velocity (PWV) was measured in 207 hypertensive patients (average age, 63 ± 8 years). To measure CMV pp65 and IE-1-specific T-cell responses, we performed intracellular cytokine staining (ICS) and enzyme-linked immunospot (ELISPOT) assays. We also analyzed CMV-specific T-cell responses against 10 different CMV antigens using ELISPOT assays.

Results
In patients with hypertension, senescent CD8T-cell frequencies were significantly correlated with arterial stiffness. Moreover, arterial stiffness was independently associated with CMV pp65-specific CD8T-cell responses as measured by ICS. CMV-specific CD8T-cell responses measured by ICS and ELISPOT assays showed good agreement and significant correlation with each other. ELISPOT analyses against 10 different CMV antigens revealed a consistent response pattern irrespective of age, gender, and diabetes.

Conclusions
CMV pp65-specific CD8T-cell responses were independently correlated with arterial stiffness in patients with hypertension. Additionally, the results of ICS and ELISPOT assays showed a significant correlation and good agreement with each other. These findings are important for guiding choices regarding the broad clinical application of CMV-specific T-cell response assays in this patient population.

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