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Analysis of the association of non-synonymous polymorphisms in ADH genes with hazardous drinking in HIV-1 positive individuals.

Analysis of the association of non-synonymous polymorphisms in ADH genes with hazardous drinking in HIV-1 positive individuals.
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Wolf JM, Simon D, Béria JU, Tietzmann DC, Stein AT, Lunge VR,


Wolf JM, Simon D, Béria JU, Tietzmann DC, Stein AT, Lunge VR, (click to view)

Wolf JM, Simon D, Béria JU, Tietzmann DC, Stein AT, Lunge VR,

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Alcoholism, clinical and experimental research 2017 08 23() doi 10.1111/acer.13486

Abstract
BACKGROUND
Hazardous drinking (HD) is a serious health problem in people infected with human immunodeficiency virus type 1 (HIV-1). Single nucleotide polymorphisms (SNPs) in alcohol dehydrogenase (ADH) genes have been associated with HD in different populations, but there was no data about this in HIV-1 positive individuals. This study investigated the association of four non-synonymous SNPs in ADH genes (Arg48His and Arg370Cys in ADH1B gene; Arg272Gln and Ile350Val in ADH1C gene) with HD in people living with HIV-1.

METHODS
This case-control study included 365 HIV-1 positive individuals (121 with HD and 244 without HD). Socio-demographic variables were collected with a standardized individual questionnaire. HD (score ≥8) and binge drinking (BD) (drinks on the same occasion ≥5) were detected with the Alcohol Use Disorders Identification Test (AUDIT). The four SNPs were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Adjusted odds ratios (aOR) and 95% confidence intervals (CIs) were calculated using logistic regression analysis. The Bonferroni correction was used (considering the four SNPs studied).

RESULTS
There were not significant differences in the frequencies of Arg370Cys, Arg272Gln, and Ile350Val polymorphisms between HD cases and controls. Otherwise, Arg/His genotype (rs1229984) in ADH1B gene showed a protective effect against HD (aOR = 0.36; 95% CI: 0.14-0.90) and BD (aOR = 0.49; 95% CI: 0.21-0.95). Nevertheless, these differences were no longer significant after Bonferroni correction.

CONCLUSIONS
The results of this study suggest a possible effect of the Arg48His genotype on the protection against HD in HIV-1 positive individuals. This article is protected by copyright. All rights reserved.

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