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Analysis of the relationship between interleukin polymorphisms within miRNA-binding regions and alcoholic liver disease.

Analysis of the relationship between interleukin polymorphisms within miRNA-binding regions and alcoholic liver disease.
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Novo-Veleiro I, Cieza-Borrella C, Pastor I, González-Sarmiento R, Laso FJ, Marcos M,


Novo-Veleiro I, Cieza-Borrella C, Pastor I, González-Sarmiento R, Laso FJ, Marcos M, (click to view)

Novo-Veleiro I, Cieza-Borrella C, Pastor I, González-Sarmiento R, Laso FJ, Marcos M,

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Revista clinica espanola 2018 03 19() pii 10.1016/j.rce.2018.02.005

Abstract
INTRODUCTION
Alcohol consumption promotes inflammation through the Toll-like receptor 4 (TLR4)/nuclear factor (NF)-κB pathway, leading to organic damage. Some micro-RNA (miRNA) molecules modulate this inflammatory response by downregulating TLR4/NF-κB pathway mediators, like interleukins (ILs). Thus, polymorphisms within IL genes located near miRNA binding sites could modify the risk of ethanol-induced damage. The present study analyzed potential relationships between alcoholism or alcoholic liver disease (ALD) and IL12B 2124 G>T (rs1368439), IL16 5000 C>T (rs1131445), IL1R1 3114 C>T (rs3917328), and NFKB1 3400 A>G (rs4648143) polymorphisms.

PATIENTS AND METHODS
The study included 301 male alcoholic patients and 156 male healthy volunteers. Polymorphisms were genotyped using TaqManPCR assays for allelic discrimination. Allele and genotype frequencies were compared between groups. Logistic regression analysis was performed to analyze the inheritance model.

RESULTS
Analysis of the IL1R1 (rs3917328) polymorphism showed that the proportion of alleleT carriers (CT and TT genotypes) was higher in healthy controls (9.7%) than in alcoholic patients (6.5%; P=.042). However, multivariable logistic regression analyses did not yield a significant result. No differences between groups were found for other analyzed polymorphisms.

CONCLUSIONS
Our study describes, for the first time, the expected frequencies of certain polymorphisms within miRNA-binding sites in alcoholic patients with and without ALD. Further studies should be developed to clarify the potential relevance of these polymorphisms in alcoholism and ALD development.

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