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ARCHITECT HIV Combo Ag/Ab® and RealTime® HIV-1 assays detect diverse HIV strains in clinical specimens.

ARCHITECT HIV Combo Ag/Ab® and RealTime® HIV-1 assays detect diverse HIV strains in clinical specimens.
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Rodgers MA, Vallari AS, Yamaguchi J, Holzmayer V, Harris B, Toure-Kane C, M'Boup S, Badreddine S, McArthur C, Ndembi N, Mbanya DN, Kaptué L, Cloherty G,


Rodgers MA, Vallari AS, Yamaguchi J, Holzmayer V, Harris B, Toure-Kane C, M'Boup S, Badreddine S, McArthur C, Ndembi N, Mbanya DN, Kaptué L, Cloherty G, (click to view)

Rodgers MA, Vallari AS, Yamaguchi J, Holzmayer V, Harris B, Toure-Kane C, M'Boup S, Badreddine S, McArthur C, Ndembi N, Mbanya DN, Kaptué L, Cloherty G,

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AIDS research and human retroviruses 2017 12 12() doi 10.1089/AID.2017.0244

Abstract

Periodic evaluation of the impact of viral diversity on diagnostic tests is critical to ensure current technologies are keeping pace with viral evolution. To determine whether HIV diversity impacts the ARCHITECT HIV Combo Ag/Ab (HIV Combo) or RealTime HIV-1 (RT) assays, a set of N=199 HIV clinical specimens from Cameroon, Senegal, Saudi Arabia, and Thailand were sequenced and tested in both assays. The panel included historical Group N and P specimens and a newly identified Group N specimen. These and specimens classified as H, U/URF, CRF01, 02, 06, 09, 11, 13, 18, 22, 37, and 43 were detected by both the RT assay (1.75-6.84 log copies/ml) and the HIV Combo assay (3.26-1121.96 sample to cutoff ratios). Sequence alignment identified 3 or fewer mismatches to the RT assay oligos in 82.4% of samples. Altogether, these data demonstrate the HIV Combo and RT assays detect diverse strains of HIV in clinical specimens.

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