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Artificial Auricular Cartilage Using Silk Fibroin and Polyvinyl Alcohol Hydrogel.

Artificial Auricular Cartilage Using Silk Fibroin and Polyvinyl Alcohol Hydrogel.
Author Information (click to view)

Lee JM, Sultan MT, Kim SH, Kumar V, Yeon YK, Lee OJ, Park CH,


Lee JM, Sultan MT, Kim SH, Kumar V, Yeon YK, Lee OJ, Park CH, (click to view)

Lee JM, Sultan MT, Kim SH, Kumar V, Yeon YK, Lee OJ, Park CH,

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International journal of molecular sciences 2017 08 0418(8) pii E1707
Abstract

Several methods for auricular cartilage engineering use tissue engineering techniques. However, an ideal method for engineering auricular cartilage has not been reported. To address this issue, we developed a strategy to engineer auricular cartilage using silk fibroin (SF) and polyvinyl alcohol (PVA) hydrogel. We constructed different hydrogels with various ratios of SF and PVA by using salt leaching, silicone mold casting, and freeze-thawing methods. We characterized each of the hydrogels in terms of the swelling ratio, tensile strength, pore size, thermal properties, morphologies, and chemical properties. Based on the cell viability results, we found a blended hydrogel composed of 50% PVA and 50% SF (P50/S50) to be the best hydrogel among the fabricated hydrogels. An intact 3D ear-shaped auricular cartilage formed six weeks after the subcutaneous implantation of a chondrocyte-seeded 3D ear-shaped P50/S50 hydrogel in rats. We observed mature cartilage with a typical lacunar structure both in vitro and in vivo via histological analysis. This study may have potential applications in auricular tissue engineering with a human ear-shaped hydrogel.

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