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Bacteriophage T5 gene D10 encodes a branch-migration protein.

Bacteriophage T5 gene D10 encodes a branch-migration protein.
Author Information (click to view)

Wong IN, Sayers JR, Sanders CM,


Wong IN, Sayers JR, Sanders CM, (click to view)

Wong IN, Sayers JR, Sanders CM,

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Scientific reports 2016 12 236() 39414 doi 10.1038/srep39414
Abstract

Helicases catalyze the unwinding of double-stranded nucleic acids where structure and phosphate backbone contacts, rather than nucleobase sequence, usually determines substrate specificity. We have expressed and purified a putative helicase encoded by the D10 gene of bacteriophage T5. Here we report that this hitherto uncharacterized protein possesses branch migration and DNA unwinding activity. The initiation of substrate unwinding showed some sequence dependency, while DNA binding and DNA-dependent ATPase activity did not. DNA footprinting and purine-base interference assays demonstrated that D10 engages these substrates with a defined polarity that may be established by protein-nucleobase contacts. Bioinformatic analysis of the nucleotide databases revealed genes predicted to encode proteins related to D10 in archaebacteria, bacteriophages and in viruses known to infect a range of eukaryotic organisms.

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