Advertisement

 

 

Bacteriophages are more virulent to bacteria with human cells than they are in bacterial culture; insights from HT-29 cells.

Bacteriophages are more virulent to bacteria with human cells than they are in bacterial culture; insights from HT-29 cells.
Author Information (click to view)

Shan J, Ramachandran A, Thanki AM, Vukusic FBI, Barylski J, Clokie MRJ,


Shan J, Ramachandran A, Thanki AM, Vukusic FBI, Barylski J, Clokie MRJ, (click to view)

Shan J, Ramachandran A, Thanki AM, Vukusic FBI, Barylski J, Clokie MRJ,

Advertisement

Scientific reports 2018 03 238(1) 5091 doi 10.1038/s41598-018-23418-y
Abstract

Bacteriophage therapeutic development will clearly benefit from understanding the fundamental dynamics of in vivo phage-bacteria interactions. Such information can inform animal and human trials, and much can be ascertained from human cell-line work. We have developed a human cell-based system using Clostridium difficile, a pernicious hospital pathogen with limited treatment options, and the phage phiCDHS1 that effectively kills this bacterium in liquid culture. The human colon tumorigenic cell line HT-29 was used because it simulates the colon environment where C. difficile infection occurs. Studies on the dynamics of phage-bacteria interactions revealed novel facets of phage biology, showing that phage can reduce C. difficile numbers more effectively in the presence of HT-29 cells than in vitro. Both planktonic and adhered Clostridial cell numbers were successfully reduced. We hypothesise and demonstrate that this observation is due to strong phage adsorption to the HT-29 cells, which likely promotes phage-bacteria interactions. The data also showed that the phage phiCDHS1 was not toxic to HT-29 cells, and phage-mediated bacterial lysis did not cause toxin release and cytotoxic effects. The use of human cell lines to understand phage-bacterial dynamics offers valuable insights into phage biology in vivo, and can provide informative data for human trials.

Submit a Comment

Your email address will not be published. Required fields are marked *

19 − four =

[ HIDE/SHOW ]