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Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum.

Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum.
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Chu CH, Sarangadharan I, Regmi A, Chen YW, Hsu CP, Chang WH, Lee GY, Chyi JI, Chen CC, Shiesh SC, Lee GB, Wang YL,


Chu CH, Sarangadharan I, Regmi A, Chen YW, Hsu CP, Chang WH, Lee GY, Chyi JI, Chen CC, Shiesh SC, Lee GB, Wang YL, (click to view)

Chu CH, Sarangadharan I, Regmi A, Chen YW, Hsu CP, Chang WH, Lee GY, Chyi JI, Chen CC, Shiesh SC, Lee GB, Wang YL,

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Scientific reports 2017 07 127(1) 5256 doi 10.1038/s41598-017-05426-6

Abstract

In this study, a new type of field-effect transistor (FET)-based biosensor is demonstrated to be able to overcome the problem of severe charge-screening effect caused by high ionic strength in solution and detect proteins in physiological environment. Antibody or aptamer-immobilized AlGaN/GaN high electron mobility transistors (HEMTs) are used to directly detect proteins, including HIV-1 RT, CEA, NT-proBNP and CRP, in 1X PBS (with 1%BSA) or human sera. The samples do not need any dilution or washing process to reduce the ionic strength. The sensor shows high sensitivity and the detection takes only 5 minutes. The designs of the sensor, the methodology of the measurement, and the working mechanism of the sensor are discussed and investigated. A theoretical model is proposed based on the finding of the experiments. This sensor is promising for point-of-care, home healthcare, and mobile diagnostic device.

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