Advertisement

 

 

Blood neuron-derived exosomes as biomarkers of cognitive impairment in HIV.

Blood neuron-derived exosomes as biomarkers of cognitive impairment in HIV.
Author Information (click to view)

Sun B, Dalvi P, Abadjian L, Tang N, Pulliam L,


Sun B, Dalvi P, Abadjian L, Tang N, Pulliam L, (click to view)

Sun B, Dalvi P, Abadjian L, Tang N, Pulliam L,

Advertisement
Share on FacebookTweet about this on TwitterShare on LinkedIn

AIDS (London, England) 31(14) F9-F17 doi 10.1097/QAD.0000000000001595

Abstract
OBJECTIVE
To investigate proteins associated with neuronal damage in plasma neuron-derived exosomes (NDE) of HIV-infected study participants as a liquid biomarker for cognitive impairment.

METHODS
Plasma NDE were isolated using precipitation and immunoadsorption with antibody to a cell surface-specific neuronal marker. Total exosomes and NDE were enumerated, characterized, and proteins extracted and targets quantified by ELISA.

RESULTS
Plasma NDE from 23 HIV seropositive individuals of which 11 had mild cognitive impairment, and 12 HIV seronegative controls of which three had cognitive impairment were isolated. NDE were enriched for the neuronal markers neurofilament light (NF-L) and synaptophysin (SYP). Neuropsychologically impaired individuals had fewer NDE compared with neuropsychologically normal study participants. NDE from neuropsychologically impaired study participants had significantly higher levels of high-mobility group box 1 (HMGB1), NF-L, and amyloid β proteins compared with neuropsychologically normal individuals. NDE HMGB1 protein significantly decreased with age in HIV-infected individuals.

CONCLUSION
Plasma NDE were altered in several ways in HIV infection. Elevated HMGB1, NF-L, and amyloid β proteins could distinguish cognitive impairment. NDE contents reflect neuronal health in ‘real time’ and may be useful for following cognitive impairment and response to therapy in HIV infection.

Submit a Comment

Your email address will not be published. Required fields are marked *

17 − eight =

[ HIDE/SHOW ]