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The following is a summary of “Analysis of Antifungal Drug Resistance Among Candida Spp. and Other Pathogenic Yeasts Isolated from Patients in Eastern Poland: Diagnostic Problems,” published in the April 2025 issue of Infection and Drug Resistance by Olender et al.
Researchers conducted a retrospective study to examine the distribution of Candida species and evaluate their antifungal susceptibility, including resistance patterns in rare clinically relevant strains.
They analyzed 197 yeast isolates using biochemical methods and matrix-assisted laser desorption ionization–time of flight (MALDI-TOF). Minimum inhibitory concentrations (MICs) were determined for amphotericin B, fluconazole, itraconazole, voriconazole, isavuconazole, posaconazole, caspofungin, micafungin, and anidulafungin. The result interpretation was followed by the European Committee on Antimicrobial Susceptibility Testing (EUCAST), Clinical and Laboratory Standards Institute (CLSI) guidelines, and relevant published literature.
The results showed that the examined yeast isolates included Candida albicans (n=78), Candida glabrata (Nakaseomyces glabrata) (n=30), Candida dubliniensis (n=23), Candida krusei (Pichia kudriavzevii) (n=13), Candida parapsilosis (n=13), Candida tropicalis (n=7), Candida kefyr (Kluyveromyces marxianus) (n=6), Candida lusitaniae (Clavispora lusitaniae) (n=6), Candida lipolytica (Yarrowia lipolytica) (n=3), Candida famata (Debaryomyces hansenii) (n=2), Candida intermedia (n=2), Candida guillermondii (Meyerozyma guilliermondii) (n=2), Candida ciferrii (n=1), Candida orthopsilosis (n=1), Candida pelliculosa (Wickerhamomyces anomalus) (n=1), Candida shehatae (n=1), Candida fabianii (Cyberlindnera fabianii) (n=1), Cryptococcus humicola (Vanrija humicola) (n=4), and Saccharomyces cerevisiae (n=3). The highest proportions of antifungal resistance were found in C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, and C. lusitaniae.
Investigators concluded that the identification of rare Candida species among the studied isolates, which can be difficult in routine diagnostics and often requires MALDI-TOF MS, and the broad range of isolated species, potentially complicating targeted antifungal therapy due to absent reference MIC ranges, highlights the value of gradient strips as an accurate, reproducible, and convenient method for MIC determination.
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