Advertisement

 

 

Cardiomyocyte oxidants production may signal to T. cruzi intracellular development.

Cardiomyocyte oxidants production may signal to T. cruzi intracellular development.
Author Information (click to view)

Dias PP, Capila RF, do Couto NF, Estrada D, Gadelha FR, Radi R, Piacenza L, Andrade LO,


Dias PP, Capila RF, do Couto NF, Estrada D, Gadelha FR, Radi R, Piacenza L, Andrade LO, (click to view)

Dias PP, Capila RF, do Couto NF, Estrada D, Gadelha FR, Radi R, Piacenza L, Andrade LO,

Advertisement

PLoS neglected tropical diseases 2017 08 2311(8) e0005852 doi 10.1371/journal.pntd.0005852
Abstract

Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, presents a variable clinical course, varying from asymptomatic to serious debilitating pathologies with cardiac, digestive or cardio-digestive impairment. Previous studies using two clonal T. cruzi populations, Col1.7G2 (T. cruzi I) and JG (T. cruzi II) demonstrated that there was a differential tissue distribution of these parasites during infection in BALB/c mice, with predominance of JG in the heart. To date little is known about the mechanisms that determine this tissue selection. Upon infection, host cells respond producing several factors, such as reactive oxygen species (ROS), cytokines, among others. Herein and in agreement with previous data from the literature we show that JG presents a higher intracellular multiplication rate when compared to Col1.7G2. We also showed that upon infection cardiomyocytes in culture may increase the production of oxidative species and its levels are higher in cultures infected with JG, which expresses lower levels of antioxidant enzymes. Interestingly, inhibition of oxidative stress severely interferes with the intracellular multiplication rate of JG. Additionally, upon H2O2-treatment increase in intracellular Ca2+ and oxidants were observed only in JG epimastigotes. Data presented herein suggests that JG and Col1.7G2 may sense extracellular oxidants in a distinct manner, which would then interfere differently with their intracellular development in cardiomyocytes.

Submit a Comment

Your email address will not be published. Required fields are marked *

nineteen + 18 =

[ HIDE/SHOW ]