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Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer.

Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer.
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Wang N, Song X, Liu L, Niu L, Wang X, Song X, Xie L,


Wang N, Song X, Liu L, Niu L, Wang X, Song X, Xie L, (click to view)

Wang N, Song X, Liu L, Niu L, Wang X, Song X, Xie L,

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Cancer science 2018 03 23() doi 10.1111/cas.13581
Abstract

This study aimed to investigate the overall changes in exosomal proteome in metastatic and nonmetastatic non-small-cell lung cancers (NSCLCs) and healthy human serum samples, and evaluate the potential of serum exosomal biomarkers to predict the NSCLC metastasis. Tandem mass tags combined with multidimensional liquid chromatography and mass spectrometry analysis were used for screening the proteomic profiles of the serum samples. The quantitative proteome, significant pathway, and functional categories of patients with metastatic and nonmetastatic NSCLCs and healthy donors were investigated. In total 552 proteins of the 628 protein groups identified were quantified. Bioinformatic analysis revealed that the quantifiable proteins were mainly involved in multiple biological functions, metastasis-related pathways. Moreover, the lipopolysaccharide-binding proteins (LBPs) in the exosomes were found to be well distinguished between patients with metastatic and patients with nonmetastatic NSCLC. The area under the curve (AUC) was 0.803 with a sensitivity of 83.1% and specificity of 67% (P < 0.0001). The circulating LBPs were also well distinguishable between metastatic and nonmetastatic NSCLCs, the AUC was 0.683 with a sensitivity of 79.5% and specificity of 47.2% (P = 0.005). This novel study provided a reference proteome map for metastatic NSCLC. Patients with metastatic and nonmetastatic NSCLCs differed in exosome-related proteins in the serum. The LBPs might be promising and effective candidates of metastatic NSCLC. This article is protected by copyright. All rights reserved.

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