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Clinical proteomic analysis of scrub typhus infection.

Clinical proteomic analysis of scrub typhus infection.
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Park EC, Lee SY, Yun SH, Choi CW, Lee H, Song HS, Jun S, Kim GH, Lee CS, Kim SI,


Park EC, Lee SY, Yun SH, Choi CW, Lee H, Song HS, Jun S, Kim GH, Lee CS, Kim SI, (click to view)

Park EC, Lee SY, Yun SH, Choi CW, Lee H, Song HS, Jun S, Kim GH, Lee CS, Kim SI,

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Clinical proteomics 2018 02 1415() 6 doi 10.1186/s12014-018-9181-5
Abstract
Background
Scrub typhus is an acute and febrile infectious disease caused by the Gram-negative α-proteobacteriumfrom the family Rickettsiaceae that is widely distributed in Northern, Southern and Eastern Asia. In the present study, we analysed the serum proteome of scrub typhus patients to investigate specific clinical protein patterns in an attempt to explain pathophysiology and discover potential biomarkers of infection.

Methods
Serum samples were collected from three patients (before and after treatment with antibiotics) and three healthy subjects. One-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by liquid chromatography-tandem mass spectrometry was performed to identify differentially abundant proteins using quantitative proteomic approaches. Bioinformatic analysis was then performed using Ingenuity Pathway Analysis.

Results
Proteomic analysis identified 236 serum proteins, of which 32 were differentially expressed in normal subjects, naive scrub typhus patients and patients treated with antibiotics. Comparative bioinformatic analysis of the identified proteins revealed up-regulation of proteins involved in immune responses, especially complement system, following infection with, and normal expression was largely rescued by antibiotic treatment.

Conclusions
This is the first proteomic study of clinical serum samples from scrub typhus patients. Proteomic analysis identified changes in protein expression upon infection withand following antibiotic treatment. Our results provide valuable information for further investigation of scrub typhus therapy and diagnosis.

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