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[Comparative study of HIV-1/2 antibody confirmatory assay: Geenius™ versus INNO-LIA™].

[Comparative study of HIV-1/2 antibody confirmatory assay: Geenius™ versus INNO-LIA™].
Author Information (click to view)

Aguinaga A, Navascués A, Polo I, Ezpeleta C,


Aguinaga A, Navascués A, Polo I, Ezpeleta C, (click to view)

Aguinaga A, Navascués A, Polo I, Ezpeleta C,

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Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia 2016 12 23() pii

Abstract
OBJECTIVE
The aim of the study is to compare two confirmatory tests for HIV-1/2 infection.

METHODS
A prospective study was carried out between 01/01/2015 and 12/31/2015. Serum samples with repeatedly positive results in the Antibody-Antigen-HIV-1/2 (Architect, Abbott) screening assay were included. The serum samples corresponding to new diagnosed cases were selected and were used to compare the two confirmatory assays: Geenius™ HIV-1/2 (Bio-Rad) and INNO-LIA™ HIV-1/2 score line-immunoassay (Innogene-tics®). The HIV-1 viral load (Cobas® AmpliPrepHIV, Ro-che) was performed in discordant or indeterminate cases.

RESULTS
Eight five samples were included. The results of both confirmatory assays were concordant in 80/85 samples: 53 HIV-1, 1 HIV-2, 25 negative and one indeterminate. Cohen’s Kappa concordance coefficient between Geenius™ and INNO-LIA™ techniques was very high (0.878).

CONCLUSIONS
The concordance between the two assays is high. The procedure for Geenius™ is simple and fast. Geenius™ is a good alternative to include in the HIV-1/2 diagnostic algorithm.

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