The family of vibrational spectroscopic imaging techniques grows every few years and there is a need to compare and contrast new modalities with the better understood ones, especially in the case of demanding biological samples. Three vibrational spectroscopy techniques (High Definition FT-IR, Raman and AFM-IR) were applied for subcellular chemical imaging of cholesteryl esters in PC-3 prostate cancer cells. The techniques were compared and contrasted in terms of image quality, spectral pattern, and chemical information. All tested techniques were found to be useful in chemical imaging of cholesterol derivatives in cancer cells. The results obtained from FT-IR and Raman imaging showed to be comparable, whereas those achieved from AFM-IR study exhibited higher spectral heterogeneity. It confirms AFM-IR method as a powerful tool in local chemical imaging of cells at the nanoscale level. Furthermore, due to polarization effect, p-polarized AFM-IR spectra showed strong enhancement of lipid bands when compared to FT-IR. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
November 19, 2020
Refractive and visual outcomes of small incision lenticule extraction (SMILE) in eyes with thin corneas.
November 5, 2020
October 6, 2020
- ASCO – Lung CancerASCO.20 Virtual Scientific Program, held May 29 - 31, brought professionals from all over the world together to hear the brightest minds in oncology present state-of-the-art treatment modalities and new therapies.
- AACR-2020The American Association for Cancer Research is the world's oldest and largest professional association related to cancer research.
- ACC 2020The American College of Cardiology decided to cancel ACC.20/WCC due to COVID-19, which was scheduled to take place March 28-30 in Chicago. However, ACC.20/WCC Virtual Meeting continues to release cutting edge science and practice changing updates for cardiovascular professionals on demand and free through June 2020.
- ASCO 2019The 2019 ASCO Annual Meeting, taking place May 31-June 4 in Chicago, will bring together more than 32,000 oncology professionals from across the globe. The theme of this year’s conference is Caring for Every Patient, Learning From Every Patient.