Advertisement

 

 

Computational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light.

Computational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light.
Author Information (click to view)

Daloglu MU, Ray A, Gorocs Z, Xiong M, Malik R, Bitan G, McLeod E, Ozcan A,


Daloglu MU, Ray A, Gorocs Z, Xiong M, Malik R, Bitan G, McLeod E, Ozcan A, (click to view)

Daloglu MU, Ray A, Gorocs Z, Xiong M, Malik R, Bitan G, McLeod E, Ozcan A,

Advertisement
Share on FacebookTweet about this on TwitterShare on LinkedIn

Scientific reports 2017 03 097() 44157 doi 10.1038/srep44157

Abstract

Significant progress in characterization of nanoparticles and biomolecules was enabled by the development of advanced imaging equipment with extreme spatial-resolution and sensitivity. To perform some of these analyses outside of well-resourced laboratories, it is necessary to create robust and cost-effective alternatives to existing high-end laboratory-bound imaging and sensing equipment. Towards this aim, we have designed a holographic on-chip microscope operating at an ultraviolet illumination wavelength (UV) of 266 nm. The increased forward scattering from nanoscale objects at this short wavelength has enabled us to detect individual sub-30 nm nanoparticles over a large field-of-view of >16 mm(2) using an on-chip imaging platform, where the sample is placed at ≤0.5 mm away from the active area of an opto-electronic sensor-array, without any lenses in between. The strong absorption of this UV wavelength by biomolecules including nucleic acids and proteins has further enabled high-contrast imaging of nanoscopic aggregates of biomolecules, e.g., of enzyme Cu/Zn-superoxide dismutase, abnormal aggregation of which is linked to amyotrophic lateral sclerosis (ALS) – a fatal neurodegenerative disease. This UV-based wide-field computational imaging platform could be valuable for numerous applications in biomedical sciences and environmental monitoring, including disease diagnostics, viral load measurements as well as air- and water-quality assessment.

Submit a Comment

Your email address will not be published. Required fields are marked *

one × 3 =

[ HIDE/SHOW ]