Hellenic journal of cardiology : HJC = Hellenike kardiologike epitheorese 2017 02 14() pii S1109-9666(17)30010-6
To study the correlation between GP78 gene polymorphism and blood fat, blood glucose, blood pressure as well as coronary atherosclerotic heart disease.
72 patients with coronary atherosclerotic heart disease were selected as the observation group. 68 healthy participants were selected as the control group. The gp78 gene polymorphism of both groups was studied by the method of polymerase chain reaction-restriction fragment length polymorphism (RFLP). At the same time the multiple expression quantities of GP78 gene in the tissues of both groups were tested by fluorogenic quantitative PCR, enzyme-linked immunosorbent assay (ELISA) and Western-blotting assay. Furthermore, blood fat, blood glucose and blood pressure of subjects in both groups were tested.
The percentages of gp78 gene polymorphism of Arg/Arg, Arg/Gly and Gly/Gly at the 145 locus of the study subjects in the observation group were 12.3%, 43.2% and 44.5% respectively, while those in the control group were 74.3%, 11.2% and 14.5%, and there were significant differences between both groups. Through the test results of blood fat, blood glucose and blood pressure of the objects in the observation group and the control group, significant differences were found between the two groups (P<0.05). CONCLUSION
There is significant correlation between the 145 locus of gp89 gene and coronary atherosclerotic heart disease, indexes of blood fat, blood glucose and blood pressure.