For a study, researchers sought to understand that the standard COVID-19 conclusion is affirmed by utilizing ongoing qPCR.  The study aimed to decide the relationship between the study of disease transmission, clinical attributes, the seriousness of affirmed COVID-19 cases, and Cycle threshold (Ct) values. An observational, insightful, cross-sectional review. All kids with COVID-19 under 18 years of age confessed to the Ricardo Gutiérrez Children’s Hospital between March 1, 2020, and February 28, 2021, were incorporated. The SARS-CoV-2 disease was affirmed utilizing RT-qPCR. The middle time of patients was 7 years. Ct values were assessed in 419 cases; middle Ct esteem was 23.5 [interquartile range (IQR): 18.9-30.9]. Levels were altogether lower in suggestive than asymptomatic patients (Ct: 22.1; IQR: 18.4-22.1), in youngsters less than 2 years old (Ct: 20.6; IQR: 17.3-27.3), and when test assortment was less than 4 days after side effect beginning (Ct: 21.1; IQR: 18.1-27.5). In kids more than 2 years old, Ct values were fundamentally lower in suggestive (Ct: 22.6; IQR: 18.7-29.3) than asymptomatic (Ct: 31.2; IQR: 24.5-33.3) patients. Children under 2 years old who have COVID-19 had lower Ct upsides, which acted as a proxy for increased viral burden, compared to children with more years of experience. Ct levels in indicative children older than 2 years old were lower than those seen in asymptomatic children.