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Curcumin Attenuates High Glucose-Induced Inflammatory Injury through ROS-PI3K/AKT-NF-κB Signaling Pathway in Rat Thoracic Aorta Endothelial Cells.

Curcumin Attenuates High Glucose-Induced Inflammatory Injury through ROS-PI3K/AKT-NF-κB Signaling Pathway in Rat Thoracic Aorta Endothelial Cells.
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Zhang Z, Li K,


Zhang Z, Li K, (click to view)

Zhang Z, Li K,

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Journal of diabetes investigation 2017 10 28() doi 10.1111/jdi.12767
Abstract
AIMS
Endothelial cells inflammatory injury is likely required for barrier dysfunction under hyperglycemia condition. Curcumin (CUR) is famous for its anti-inflammatory effect. However, exert few reports about the anti-inflammatory effect of CUR induced by high glucose in endothelial cells. The aim of the present study is to investigate the inflammatory effect of high glucose and the anti-inflammatory effect of CUR induced by high glucose in rat thoracic aorta endothelial cells (TAECs).

MATERIALS AND METHODS
A well characteristic TAECs was established and cell viability was assayed by CCK-8 method, mRNA and protein expression was identified by Real time-PCR, western blot or Elisa, respectively. The production of reactive oxygen species (ROS) was observed by a fluorescence microscope.

RESULTS
High glucose (30 mmol/L) significantly decreased the cell viability of TAECs after co-cultivated for 12 h and showed a time-dependent manner, and increased IL-1β, IL-6 and TNF-α secretion in TAECs. The injury effect of high glucose involves in ROS-PI3K/AKT-NF-κB signaling pathway. For the reason that antioxidant NAC, PI3K and NF-κB specific pathway inhibitors can abolish the secretion of this inflammatory factors; pretreatment with antioxidant NAC significantly decreased PI3K expression, the level of phosphorylated AKT and nuclear NF-κB; pretreatment of LY294002 can significantly decrease NF-κB level in nuclei. After treatment with CUR for 12 h, IL-1β, IL-6 and TNF-α secretion were markedly decreased, and PI3K expression, the phosphorylation of AKT and nuclear NF-κB level were also decreased.

CONCLUSION
Curcumin attenuates high glucose-induced inflammatory injury through ROS-PI3K/AKT-NF-κB signaling pathway in rat thoracic aorta endothelial cells. This article is protected by copyright. All rights reserved.

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