GST belongs to the class of phase II detoxification enzyme. It plays an important role in preventing damage due to reactive water-soluble compounds generated by the association of reactive intermediates with glutathione.
This study analyzed the frequencies of GSTP1 polymorphism among the Iraqi population using the PCR–RFLP technique. 100 total samples were obtained from bronchial asthma patients and from control cases were subjected to conventional PCR and RFLP to detect GSTP1 genotype and measured different parameters together such as IgE, eosinophilic count, WBC, and so forth. The GSTP1 polymorphism was determined and the 176-bp fragment was subjected to RFLP and digested with BsamA1 to recognize the A–G transition at nucleotide.
Homozygotes for the Ile105 encoding allele resulted in a 176-bp fragment found in 62% andVal105 encoding allele had two fragments of 91 and 85 bp in PCR was found in 4% of asthmatic patients.
The findings of this study provided a description of the role played by GSTP1 polymorphism in bronchial asthma among Iraqi patients. Though the study outcomes do not support the large role played by GSTP1 gene polymorphism in the evolution of bronchial asthma disorder.
Reference: https://clinicalmolecularallergy.biomedcentral.com/articles/10.1186/s12948-019-0108-0
