Preeclampsia is a common pregnancy complication that is characterized by the damage of other organs and organ systems during the period of pregnancy. Decreased placental IL9 and IL9R in preeclampsia was observed to impair trophoblast cell proliferation, invasion, and angiogenesis. This study was done with the purpose to investigate IL9 and IL9R expression in preeclampsia and assess their effects on trophoblast biological behaviors.
IL9 and IL9R expression of placenta tissue were evaluated by immunochemistry and q-PCR. Using transwell, CCK-8, and tubule formation assays measured invasion, proliferation, and angiogenesis of trophoblast with adding IL9 or anti-IL9R antibody.
IL9 and IL9R levels were significantly decreased in preeclampsia therefore it can be a predicting factor. IL9 improved trophoblast activities. Blocking IL9/IL9R resulted in decreased proliferation, invasion, and tube-formation capability of trophoblast.
The findings of this study concluded that the IL9 and IL9R contribute to the pathogenesis of preeclampsia. IL9/IL9R signaling provides a new potential therapeutic target for preventing preeclampsia. These findings can be helpful in the clinical settings and for the campaigns that are working for the prediction and prevention of preeclampsia.