For a study, Butyrate generated by gut microbiota had many beneficial impacts on the host health, and oligosaccharides emerged from host diets and arose from host mucus were an important source of its production. A considerable decrease of butyrate-producing bacteria was noted in the patients along with inflammatory bowel diseases and colorectal cancer. Though gut butyrate levels were crucial for host health, oligosaccharide metabolic characteristics in the butyrate producers were poorly characterized. The study of metabolic characteristics of fructooligosaccharides (FOSs) and other prebiotic oligosaccharides (i e. Raffinose and xylooligosaccharides; XOSs) in gut butyrate producers. 1- ketosis (ketosis) and nystose, FOSs with degrees of polymerization of 3 and 4, respectively, were involved. Fourteen species of butyrate creators were distributed into four different groups based on the oligosaccharide metabolic characteristics, which were group A (two species) metabolizing all the oligosaccharides tested, group F ( four species) metabolizing FOSs, and group N (four species) metabolizing none of the oligosaccharides tested. Species allowed to groups A and XR were rich in glycoside hydrolase (GH) holders, while those in groups of F and N were opposing. In all, 17 enzymes that were allowed to GH32 were identified in 9 of the 14 butyrate creators tested, and the variety that metabolized FOSs had the least of one active GH32 enzyme. The GH32 enzymes were distributed into four clusters by phylogenetic analysis. Heterologous gene expression examination indicated that the GH32 enzymes in each cluster had the same FOS reduction characteristics within the clusters, which might be connected to substitution/ conservation of amino acids to constrain with the substrates in GH32 enzymes. The study gave significant knowledge to understand the effect of FOS supplementation on the activities of the gut butyrate makers.