Hypoxia and sepsis are the key concerns towards modern regenerative medicine. Oxygen generating biomaterials having antibacterial property aim to answer these concerns. Hypoxia and Sepsis promote reactive oxygen species (ROS) generation at the implant site that delays wound healing. Therefore, scaffolds with antibacterial property and oxygen-producing capacities have shown ability to promote wound healing. In the present study oxygen releasing, ciprofloxacin loaded collagen chitosan scaffold was fabricated for sustained oxygen delivery. Calcium peroxide (CPO) acted as a chemical oxygen source. Oxygen release pattern exhibited a sustained release of oxygen with uniform deposition of CPO on the scaffold. The drug release study showed a prolonged, continuous, and sustained release of ciprofloxacin. Cell culture studies depicted that scaffold has suitable cell attachment and migration properties for fibroblasts. In vivo studies performed in the skin flip model visually showed better wound healing and less necrosis. Histological studies showed the maintenance of tissue architecture and the deposition of collagen. Overall, our results demonstrated that the proposed CPO coated ciprofloxacin loaded collagen- chitosan scaffold could be a promising candidate for skin tissue engineering.
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