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Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants.

Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants.
Author Information (click to view)

Dampier W, Sullivan NT, Chung CH, Mell JC, Nonnemacher MR, Wigdahl B,


Dampier W, Sullivan NT, Chung CH, Mell JC, Nonnemacher MR, Wigdahl B, (click to view)

Dampier W, Sullivan NT, Chung CH, Mell JC, Nonnemacher MR, Wigdahl B,

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Scientific reports 2017 10 317(1) 14413 doi 10.1038/s41598-017-12612-z

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated protein 9 (Cas9), including specific guide RNAs (gRNAs), can excise integrated human immunodeficiency virus type 1 (HIV-1) provirus from host chromosomes. To date, anti-HIV-1 gRNAs have been designed to account for off-target activity, however, they seldom account for genetic variation in the HIV-1 genome within and between patients, which will be crucial for therapeutic application of this technology. This analysis tests the ability of published anti-HIV-1 gRNAs to cleave publicly available patient-derived HIV-1 sequences to inform gRNA design and provides basic computational tools to researchers in the field.

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