The primary objective of this study was to investigate all the infectious etiologies in the respiratory specimens from patients that are having cardiopulmonary deterioration. Next generation sequencing had been used for this study. In this research a total of 16 pediatric patients were admitted to PICU, who had unexpected cardiopulmonary deterioration. In order to obtain precise results 10 and 6 bronchoalveolar lavage fluid and transtracheal aspirate samples were taken respectively. There was a detection of bacteria and viral pathogens in the bronchoalveolar lavage fluid that were taken as specimens from 10 patients. Generation sequencing held in detecting additional 9 pathogens irrespective of the eighteen pathogens that were already identified with the help of usual methods. Candidate pathogens were again detected by the next-generation sequencing method in 4 of 10 patients in whom there were no pathogens initially identified by conventional methods. As a conclusion it is clear that bacterial infections and viral pathogens are common in unexpected cardiopulmonary deterioration in numerous patients.

Reference link-