Acute myocardial infarction (AMI) is the heart attack happening when the blood-flow terminated to the heart muscles. C-reactive protein (CRP) level is raising significantly in AMI patients after the onset of symptom, also found the temporal variations of CRP in plasma of AMI patient. Quantifying the concentration of CRP helps to identify the condition associated with AMI. Plasmonic Enzyme Linked Immunosorbent Assay (ELISA) was utilized here to identify CRP by the sandwich of aptamer and antibody. Bare-eye CRP detection was achieved by Plasmonic ELISA through the aggregation (blue color) of gold nanoparticle in the presence of CRP, while in the absence of CRP retains its red color (dispersion). Depends on the catalase presence on the ELISA surface, hydrogen peroxide controls gold growth and differentiates with color changes. To achieve the lowest detection limit of CRP, H O (200 μM), gold seed (0.2 μM), and streptavidin-catalase (1:500) were found optimal. The detection limit was reached at 0.25 μg/mL, while it was 0.5 μg/mL in the CRP-spiked serum. This method of detection system is easier to detect the levels of CRP and helps diagnosing AMI. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
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Subash C B Gopinath