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Development and characterization of monoclonal antibodies against nucleoprotein for diagnosis of influenza A virus.

Development and characterization of monoclonal antibodies against nucleoprotein for diagnosis of influenza A virus.
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Phuong NH, Kwak C, Heo CK, Cho EW, Yang J, Poo H,


Phuong NH, Kwak C, Heo CK, Cho EW, Yang J, Poo H, (click to view)

Phuong NH, Kwak C, Heo CK, Cho EW, Yang J, Poo H,

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Journal of microbiology and biotechnology 2018 04 12() doi 10.4014/jmb.1801.01002
Abstract

Influenza, which is a highly contagious disease caused by the influenza A virus, continues to be a major health concern worldwide. Although the accurate and early diagnosis of influenza virus infection is important for controlling the spread of this disease and rapidly initiating anti-viral therapy, the current influenza diagnostic kits are limited by their low sensitivity. In this study, we developed several new influenza nucleoprotein (NP)-specific monoclonal antibodies (mAbs) and compared their sensitivity and specificity with those of commercially available anti-NP mAbs. Three mAbs, designated M24.11, M34.3 and M34.33, exhibited higher reactivities to recombinant NP proteins and A/Puerto Rico/8/1934 (H1N1) viral lysates compared with the commercial mAbs, as assessed using enzyme-linked immunosorbent assays. M34.3 and M34.33 showed higher reactivities with A/California/04/09 (pandemic H1N1) and A/Philippines/2/82 (H3N2) viral lysates than the commercial mAbs. In contrast, M24.11 had marked reactivity with H3N2 but not with pandemic H1N1. Immunofluorescent confocal microscopy showed that the three mAbs effectively detected the presence of influenza virus in lung tissues of mice infected with A/Puerto Rico/8/1934. These results indicate that the newly developed mAbs, M34.3 and M34.33, could be useful for the development of influenza diagnostics.

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