The development of vaccinations against these novel strains in human beings causes problems for public health worldwide and is the most efficient way of preventing these virus breakouts. Prior to usage, all forms of vaccinations must be examined for potential; hence, quantitative influenza vaccinations require tests. In a single SRID (radial immuno dissemination) test, the gold standard for influenza virus antigens is evaluated, and SRID reference reagents are essential in vaccination power determinations. The researchers produced cell-based reference reagents of MDCK-grown viruses and compared them with egg-based reference reagents for the purpose of evaluating the utilisation of reference reagents developed from a cell-based influenza vaccination platform.
Cell-based candidate influenza viruses were purified from a primary liquid standard (PLS), and densitometric methods were used to estimate a hemagglutinin (HA) antigen level. For more than 10 months the created PLS might be stored at 4°C. They have also created a simple HA protein purification process for goat antiserum preparation and compared the results to those obtained utilising different production platforms with standard anti-steroidal reagents. The results of the study show that both cell-based and egg-based platforms can use these referential reagents and insignificant variations between these two platform types.
Reference: https://www.tandfonline.com/doi/full/10.1080/21645515.2020.1721223
