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Development of an athyroid mouse model using (131)I ablation after preparation with a low-iodine diet.

Development of an athyroid mouse model using (131)I ablation after preparation with a low-iodine diet.
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Oh JM, Lee HW, Kalimuthu S, Gangadaran P, Baek SH, Han MH, Hong CM, Jeong SY, Lee SW, Lee J, Ahn BC,


Oh JM, Lee HW, Kalimuthu S, Gangadaran P, Baek SH, Han MH, Hong CM, Jeong SY, Lee SW, Lee J, Ahn BC, (click to view)

Oh JM, Lee HW, Kalimuthu S, Gangadaran P, Baek SH, Han MH, Hong CM, Jeong SY, Lee SW, Lee J, Ahn BC,

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Scientific reports 2017 10 167(1) 13284 doi 10.1038/s41598-017-13772-8

Abstract

We optimized the protocol for thyroid ablation in living mice using radioactive iodine (RAI) and a low-iodine diet (LID). To examine the effect of LID on thyroid ablation, mice were randomly divided into 4 groups: Vehicle, (131)I 2.775 MBq, (131)I 5.55 MBq, and LID + (131)I 2.775 MBq. The LID group was fed a LID for up to 7 days and then mice in the (131)I 2.775, (131)I 5.55, and LID + (131)I 2.775 MBq groups were intravenously administrated with (131)I, respectively. Scintigraphy imaging with (99m)Tc pertechnetate was performed once in 2 weeks for 4 weeks. After establishment of athyroid mice, control or athyroid mice were injected with human anaplastic thyroid cancer cells co-expressing sodium iodine symporter and enhanced firefly luciferase (ARO/NF) to evaluate RAI uptake. Scintigraphy imaging with (99m)Tc pertechnetate was performed with ARO/NF tumor-bearing mice. Scintigraphy imaging showed decreased thyroid uptake in the LID + (131)I 2.775 MBq group compared to other groups. Scintigraphy images showed that tumor uptake was statically higher in athyroid mice than in control mice. These data suggest that these optimized conditions for thyroid ablation could be helpful to establish an in vivo mouse model.

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