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Dimer Interface of the Human Serotonin Transporter and Effect of the Membrane Composition.

Dimer Interface of the Human Serotonin Transporter and Effect of the Membrane Composition.
Author Information (click to view)

Periole X, Zeppelin T, Schiøtt B,


Periole X, Zeppelin T, Schiøtt B, (click to view)

Periole X, Zeppelin T, Schiøtt B,

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Scientific reports 2018 03 238(1) 5080 doi 10.1038/s41598-018-22912-7
Abstract

The oligomeric state of membrane proteins has recently emerged in many cases as having an effect on their function. However, the intrinsic dynamics of their spatial organization in cells and model systems makes it challenging to characterize. Here we use molecular dynamics (MD) simulations at multiple resolutions to determine the dimer conformation of the human serotonin transporter (hSERT). From self-assembly simulations we predict dimer candidates and subsequently quantify their relative strength. We use umbrella sampling (US) replica exchange MD simulations for which we present extensive analysis of their efficiency and improved sampling compared to regular US MD simulations. The data shows that the most stable hSERT dimer interface is symmetrical and involves transmembrane helix 12 (TM12), similar to the crystal structure of the bacterial homologue LeuT, but with a slightly different orientation. We also describe the supramolecular organization of hSERT from a 250 μs self-assembly simulation. Finally, the effects of the presence of phosphatidylinositol bisphosphate or cholesterol in the membrane model has been quantified for the TM12-TM12 predicted interface. Collectively, the presented data bring new insight to the area of protein and lipid interplay in biological membranes.

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