Advertisement

 

 

Double-negative (CD27IgD) B cells are expanded in NSCLC and inversely correlate with affinity-matured B cell populations.

Double-negative (CD27IgD) B cells are expanded in NSCLC and inversely correlate with affinity-matured B cell populations.
Author Information (click to view)

Centuori SM, Gomes CJ, Kim SS, Putnam CW, Larsen BT, Garland LL, Mount DW, Martinez JD,


Centuori SM, Gomes CJ, Kim SS, Putnam CW, Larsen BT, Garland LL, Mount DW, Martinez JD, (click to view)

Centuori SM, Gomes CJ, Kim SS, Putnam CW, Larsen BT, Garland LL, Mount DW, Martinez JD,

Advertisement

Journal of translational medicine 2018 02 1516(1) 30 doi 10.1186/s12967-018-1404-z

Abstract
BACKGROUND
The presence of B cells in early stage non-small cell lung cancer (NSCLC) is associated with longer survival, however, the role these cells play in the generation and maintenance of anti-tumor immunity is unclear. B cells differentiate into a variety of subsets with differing characteristics and functions. To date, there is limited information on the specific B cell subsets found within NSCLC. To better understand the composition of the B cell populations found in NSCLC we have begun characterizing B cells in lung tumors and have detected a population of B cells that are CD79ACD27IgD. These CD27IgD(double-negative) B cells have previously been characterized as unconventional memory B cells and have been detected in some autoimmune diseases and in the elderly population but have not been detected previously in tumor tissue.

METHODS
A total of 15 fresh untreated NSCLC tumors and 15 matched adjacent lung control tissues were dissociated and analyzed by intracellular flow cytometry to detect the B cell-related markers CD79A, CD27 and IgD. All CD79AB cells subsets were classified as either naïve (CD27IgD), affinity-matured (CD27IgD), early memory/germinal center cells (CD27IgD) or double-negative B cells (CD27IgD). Association of double-negative B cells with clinical data including gender, age, smoking status, tumor diagnosis and pathologic differentiation status were also examined using the logistic regression analysis for age and student’s t-test for all other variables. Associations with other B cell subpopulations were examined using Spearman’s rank correlation.

RESULTS
We observed that double-negative B cells were frequently abundant in lung tumors compared to normal adjacent controls (13 out of 15 cases), and in some cases made up a substantial proportion of the total B cell compartment. The presence of double-negative cells was also found to be inversely related to the presence of affinity-matured B cells within the tumor, Spearman’s coefficient of – 0.76.

CONCLUSIONS
This study is the first to observe the presence of CD27IgDdouble-negative B cells in human NSCLC and that this population is inversely correlated with traditional affinity-matured B cell populations.

Submit a Comment

Your email address will not be published. Required fields are marked *

1 × 2 =

[ HIDE/SHOW ]