Molecular nutrition & food research 2017 10 05() doi 10.1002/mnfr.201700596
We investigated whether early supplementation of precursors of hydrogen sulfide (H2 S), D- or L-cysteine can prevent hypertension and kidney damage in spontaneously hypertensive rats (SHR) treated with high-salt.
METHODS AND RESULTS
We examined 12-week-old male SHRs from four groups: SHR, high salt SHR (SHRs received 1% NaCl in drinking water for 8 weeks), high salt SHR+D (SHRs received high salt and D-cysteine), and high salt SHR+L (SHRs received high salt and L-cysteine). D- or L-cysteine was supplemented at 8 mmol/kg body weight/day between 4 and 6 weeks of ages. High salt intake exacerbated hypertension and kidney damage in SHRs, which was prevented by D- or L-cysteine supplementation. D- or L-cysteine supplementation reduced the degree of high salt-induced oxidative stress damage. Renal 3-mercaptopyruvate sulphurtransferase (3MST) protein levels and activity were reduced by D- or L-cysteine supplementation. Additionally, D- or L-cysteine supplementation reduced renal angiotensin I and angiotensin II concentrations, decreased mRNA expression of Ren, and increased protein levels of type 2 angiotensin II receptor.
Early supplementation of D- or L-cysteine before hypertension becomes evident may protect against hypertension and kidney damage in adult SHRs exposed to high salt consumption via regulation of oxidative stress, renin-angiotensin system, and H2 S-generating pathways. This article is protected by copyright. All rights reserved.