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Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood.

Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood.
Author Information (click to view)

Zhang FJ, Yang JT, Tang LH, Wang WN, Sun K, Ming Y, Muhammad KG, Zheng YF, Yan M,


Zhang FJ, Yang JT, Tang LH, Wang WN, Sun K, Ming Y, Muhammad KG, Zheng YF, Yan M, (click to view)

Zhang FJ, Yang JT, Tang LH, Wang WN, Sun K, Ming Y, Muhammad KG, Zheng YF, Yan M,

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Scientific reports 2017 08 117(1) 7995 doi 10.1038/s41598-017-08405-z
Abstract

The broad clinical acceptance of intraoperative blood salvage and its applications in cancer surgery remain controversial. Until now, a method that can safely eliminate cancer cells while preserving erythrocytes does not exist. Here, we investigated whether X-ray generated from linear accelerator irradiation at a certain dose can kill hepatocarcinoma cells while preserving erythrocytes. HepG2, SK-Hep1 or Huh7 cells were mixed into the aliquots of erythrocytes obtained from healthy volunteers. After the mixed cells were exposed to 30 Gy and 50 Gy X-rays irradiation, the viability, clonogenicity, DNA synthesis and tumorigenicity of the tumor cells were determined by the MTT assay, plate colony formation, 5-ethynyl-2′-deoxyuridine incorporation, and subcutaneous xenograft implantation into immunocompromised mice. The ATP, 2,3-DPG, free Hb, osmotic fragility, blood gas variables in erythrocytes and morphology of erythrocytes at 0 h, 12 h, 24 h, 48 h, 72 h after irradiation were analyzed. X-ray irradiation at 30 Gy effectively inhibited the viability, proliferation, and tumorigenicity of HepG2, SK-Hep1 and Huh7 cells without noticeably damaging the ability of oxygen-carrying, membrane integrity and morphology of erythrocytes. Theses results suggest that X-ray at 30 Gy irradiation might be safe to eliminate hepatocarcinoma cells while preserving erythrocytes in salvaged blood.

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