The purpose of this study was to see how bovine lactoferrin (bLF) affected cell survival, proliferation, and protective functions in intestinal epithelial cells-6 (IEC-6) treated with lipopolysaccharide (LPS). IEC-6 cell viability and proliferation were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Brdu assays, respectively. Flow cytometry was used to examine the dispersion of the cell cycle. Real-time PCR and ELISA were used to examine inflammatory cytokines. The amount of MAPK and NF- nuclear translocation was measured using a Western blot. In IEC-6 cells, bLF had dose-dependent effects on cell viability and proliferation, notably at 100 g/ml. Co-stimulation of bLF and LPS reduced the mRNA levels of IL-1, IL-6, and TNF- in IEC-6 cells compared to LPS treatments alone. The co-stimulation of bLF and LPS also reduced IL-6 and TNF- production. A 100 g/ml dosage of bovine lactoferrin might block the activation of the MAPK/NF- signal pathway caused by LPS.

Bovine lactoferrin may enhance cell survival and proliferation while also acting as an anti-inflammatory agent by inhibiting MAPK and NF- nuclear translocation activation. Preventing NEC in preterm newborns may be helped by supplementing formula with bLF.