Experimental parasitology 2018 03 27() pii 10.1016/j.exppara.2018.03.012
The influence of iridoid-anthocyanin aqueous extract of cornelian cherry fruits (CM) on hematological parameters, lymphocyte subsets and proliferation during Trichinella spiralis infection in mice was investigated. CM (100 mg/kg) was administered orally to T. spiralis-infected mice six times within a period encompassing three days prior to the infection and three days after the infection (dai). CM increased the percentage of CD3, CD4cells and CD4/CD8ratio and decreased total count of CD8and CD19splenocytes (5th dai). An increase in total count of CD4, CD3, CD19splenocytes was observed (21st dai). CM elevated the percentage of CD4cells (7th dai) and CD4/CD8ratio (21st dai) in MLN. CM increased (14th dai) and then reduced (21st dai) the percentage of CD8MLN lymphocytes and decreased total count of MLN CD8cells (21st dai) and B cells (14th dai). An activation of lymphocyte proliferation in spleen and simultaneous decrease in MLN on 5th dai was observed. An increase in red blood cells parameters (5th dai) and in leukocyte count (7th dai) was found. A rise in platelet count was noticed both on 5th and 7th dai. Moreover, the number of adult T. spiralis on 5th dai in mice receiving CM extract was lower than in the control mice. These results suggested that iridoid-anthocyanin aqueous extract of CM stimulated murine immune response during T. spiralis infection.