Advertisement

 

 

Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells.

Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells.
Author Information (click to view)

Chiang CF, Flint M, Lin JS, Spiropoulou CF,


Chiang CF, Flint M, Lin JS, Spiropoulou CF, (click to view)

Chiang CF, Flint M, Lin JS, Spiropoulou CF,

Advertisement
Share on FacebookTweet about this on TwitterShare on LinkedIn

PloS one 2016 Oct 2511(10) e0164768 doi 10.1371/journal.pone.0164768
Abstract

Andes virus (ANDV) is the major cause of hantavirus pulmonary syndrome (HPS) in South America. Despite a high fatality rate (up to 40%), no vaccines or antiviral therapies are approved to treat ANDV infection. To understand the role of endocytic pathways in ANDV infection, we used 3 complementary approaches to identify cellular factors required for ANDV entry into human lung microvascular endothelial cells. We screened an siRNA library targeting 140 genes involved in membrane trafficking, and identified 55 genes required for ANDV infection. These genes control the major endocytic pathways, endosomal transport, cell signaling, and cytoskeleton rearrangement. We then used infectious ANDV and retroviral pseudovirions to further characterize the possible involvement of 9 of these genes in the early steps of ANDV entry. In addition, we used markers of cellular endocytosis along with chemical inhibitors of known endocytic pathways to show that ANDV uses multiple routes of entry to infect target cells. These entry mechanisms are mainly clathrin-, dynamin-, and cholesterol-dependent, but can also occur via a clathrin-independent manner.

Submit a Comment

Your email address will not be published. Required fields are marked *

13 − 5 =

[ HIDE/SHOW ]