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Endurance training lowers ribosome density despite increasing ribosome biogenesis markers in rodent skeletal muscle.

Endurance training lowers ribosome density despite increasing ribosome biogenesis markers in rodent skeletal muscle.
Author Information (click to view)

Romero MA, Mobley CB, Linden MA, Meers GM, Martin JS, Young KC, Rector RS, Roberts MD,


Romero MA, Mobley CB, Linden MA, Meers GM, Martin JS, Young KC, Rector RS, Roberts MD, (click to view)

Romero MA, Mobley CB, Linden MA, Meers GM, Martin JS, Young KC, Rector RS, Roberts MD,

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BMC research notes 2017 08 1110(1) 399 doi 10.1186/s13104-017-2736-0
Abstract
OBJECTIVE
The purpose of this study was to examine if: (a) high sugar/high fat Western diet (WD)-feeding affects skeletal muscle ribosome biogenesis markers in hyperphagic, diabetic-prone Otsuka Long-Evans Tokushima Fatty (OLETF) rats, and (b) 12 weeks of treadmill training rescued potential detriments that WD feeding exerted on these markers.

METHODS
Eight week-old male OLETF rats were fed a low-fat control diet (O-CON, n = 10) or high/sucrose/cholesterol Western diet (WD). At weeks 20-32 of age, WD-fed rats were divided into WD sedentary (O-WD/SED, n = 16), or WD treadmill trained (5 days/week, 60 min/day) (O-WD/EX, n = 10) conditions.

RESULTS
Interestingly, total RNA (i.e., ribosome density) was 2.3-fold greater in O-WD/SED versus O-WD/EX rats (p = 0.003) despite levels of upstream binding factor protein, RNA polymerase I protein and pre-45S rRNA being greater in O-WD/EX rats. Ribophagy (USP10 and G3BP1) and TRAMP-exosome rRNA degradation pathway (EXOSC10 and SKIV2L2) proteins were assayed to determine if these pathways were involved with lower ribosome density in O-WD/EX rats. While USP10 was higher in O-CON versus O-WD/SED and O-WD/EX rats (p < 0.001 and p < 0.001, respectively), G3BP1, EXOSC10 and SKIV2L2 did not differ between groups. Nop56 and Ncl mRNAs, ribosome assembly markers, were highest in O-WD/EX rats. However, Fbl mRNA and 28S rRNA, downstream ribosome processing markers, were lowest in O-WD/EX rats. Collectively these data suggest that, in WD-fed rats, endurance training increases select skeletal muscle ribosome biogenesis markers. However, endurance training may reduce muscle ribosome density by interfering with rRNA processing and/or export through mechanisms independent of ribophagy or rRNA degradation.

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