Advertisement

 

 

Enhanced activation of circulating plasmacytoid dendritic cells in patients with Chronic Obstructive Pulmonary Disease and experimental smoking-induced emphysema.

Enhanced activation of circulating plasmacytoid dendritic cells in patients with Chronic Obstructive Pulmonary Disease and experimental smoking-induced emphysema.
Author Information (click to view)

Qiu SL, Kuang LJ, Tang QY, Duan MC, Bai J, He ZY, Zhang JQ, Li MH, Deng JM, Liu GN, Zhong XN,


Qiu SL, Kuang LJ, Tang QY, Duan MC, Bai J, He ZY, Zhang JQ, Li MH, Deng JM, Liu GN, Zhong XN, (click to view)

Qiu SL, Kuang LJ, Tang QY, Duan MC, Bai J, He ZY, Zhang JQ, Li MH, Deng JM, Liu GN, Zhong XN,

Advertisement
Share on FacebookTweet about this on TwitterShare on LinkedIn

Clinical immunology (Orlando, Fla.) 2017 11 07() pii S1521-6616(16)30701-X
Abstract

Plasmacytoid dendritic cells (pDCs) are key cells bridging the innate with adaptive immunity. However, the phenotypic characteristics of circulating pDCs and its role in smoking related-Chronic Obstructive Pulmonary Disease (COPD) remain largely unknown. The aim of this study was analyzed the phenotype of circulating pDCs and the expression of IFN-γ producing CD8(+)T cells and IL-17-producing CD8(+)T cells in patients with COPD by using multi-colour flow cytometry. The cytokine profiles in peripheral blood from all subjects were measured by ELISA. The influence of cigarette smoke on pDCs was evaluated in an experimental mouse model of emphysema. Circulating pDCs in patients with COPD and in mice exposed to cigarette smoke expressed high levels of co-stimulatory molecules CD40 or CD86 accompanied by exaggerated IFN-γ producing CD8(+)T cells and IL-17-producing CD8(+)T cells. In vitro, cigarette smoke directly promoted pDCs maturation and release of IFN-α, IL-6 and IL-12, subsequently inducing differentiation of IFN-γ producing CD8(+)T cells and IL-17-producing CD8(+)T cells from mouse naïve CD8(+)T cells. These data suggested that circulating pDCs display an enhanced activation phenotype in patients with COPD and in experimental smoking mouse model of emphysema, which might contribute to exaggerated IFN-γ producing CD8(+)T and IL-17-producing CD8(+)T cell-mediated immune responses.

Submit a Comment

Your email address will not be published. Required fields are marked *

one × 1 =

[ HIDE/SHOW ]