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Evaluation of a new ICT test (LDBIO Diagnostics) to detect toxoplasma IgG and IgM: comparison with the routine Architect technique.

Evaluation of a new ICT test (LDBIO Diagnostics) to detect toxoplasma IgG and IgM: comparison with the routine Architect technique.
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Mahinc C, Flori P, Delaunay E, Guillerme C, Charaoui S, Raberin H, Hafid J, L'Ollivier C,


Mahinc C, Flori P, Delaunay E, Guillerme C, Charaoui S, Raberin H, Hafid J, L'Ollivier C, (click to view)

Mahinc C, Flori P, Delaunay E, Guillerme C, Charaoui S, Raberin H, Hafid J, L'Ollivier C,

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Journal of clinical microbiology 2017 09 27() pii JCM.01106-17
Abstract

A study comparing the ICT (immunochromatography technology) Toxoplasma® IgG and IgM rapid diagnostic test (LDBIO Diagnostics, France) with the fully automated system, Architect, was performed on samples from university hospitals of Marseille and Saint-Etienne. A total of 767 prospective sera and 235 selected sera were collected. The panels were selected to test various IgG and IgM parameters. The reference technique Toxoplasma IgGII Western Blot (LDBIO Diagnostics) was used to confirm the IgG results and commercial kits Platelia Toxo IgM (Biorad) and Toxo-ISAgA (bioMérieux) were used in Saint-Etienne and Marseille respectively, as the IgM reference technique.Sensitivity and specificity of the ICT and the Architect IgG assay were compared using a prospective panel. Sensitivity was 100% for the ICT test and 92.1% for the Architect (cutoff at 1.6 IU/ml). The low IgG titer serum results confirmed that ICT sensitivity was superior to that of the Architect. Concerning the specificity, it was 98.7% (ICT) and 99.8% (Architect IgG).The ICT test is also useful to detect IgM without IgG and is both sensitive (100%) and specific (100%), as it can distinguish non-specific IgM from specific Toxoplasma IgM. Comparatively, IgM sensitivity and specificity on the Architect are 96.1% and 99.6% respectively (cutoff at 0.5 UA/ml).To conclude, this new test overcomes the limitations of the automated screening techniques, which are not sensitive enough for IgG and lack specificity for IgM (rare IgM false positive cases).

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