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Genotypic characterization of human immunodeficiency virus type 1 derived from antiretroviral drug-treated individuals residing in earthquake-affected areas in Nepal.

Genotypic characterization of human immunodeficiency virus type 1 derived from antiretroviral drug-treated individuals residing in earthquake-affected areas in Nepal.
Author Information (click to view)

Negi BS, Kotaki T, Joshi SK, Bastola A, Nakazawa M, Kameoka M,


Negi BS, Kotaki T, Joshi SK, Bastola A, Nakazawa M, Kameoka M, (click to view)

Negi BS, Kotaki T, Joshi SK, Bastola A, Nakazawa M, Kameoka M,

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AIDS research and human retroviruses 2017 03 16() doi 10.1089/AID.2017.0047

Abstract

Molecular epidemiological data on human immunodeficiency virus type 1 (HIV-1) are limited in Nepal, and has not been available in areas affected by the April 2015 earthquake. Therefore, we conducted a genotypic study on HIV-1 genes derived from individuals on antiretroviral therapy residing in 14 districts in Nepal highly affected by the earthquake. HIV-1 genomic fragments were amplified from 40 blood samples of HIV treatment-failure individuals, and a sequencing analysis was performed on these genes. In the 40 samples, 29 protease, 32 reverse transcriptase, 25 gag and 21 env genes were sequenced. HIV-1 subtyping revealed that subtype C (84.2%, 32/38) was the major subtype prevalent in the region, while CRF01_AE (7.9%, 3/38) and other recombinant forms (7.9%, 3/38) were also detected. In addition, major drug resistance mutations were identified in 21.9% (7/32) of samples, indicating the possible emergence of HIV-1 drug resistance in earthquake-affected areas in Nepal.

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